Noninvasive optical inhibition with a red-shifted microbial rhodopsin
تعليق: مضخات البروتون في العين ودورها في الإبصار--احمد شوقي محمدين 20:39، 18 أكتوبر 2016 (ت ع م)
Noninvasive optical inhibition with a red-shifted microbial rhodopsin
Amy S Chuong1–3, Mitra L Miri4,12, Volker Busskamp5,6,12, Gillian A C Matthews7,12, Leah C Acker1–3,12, Andreas T Sørensen2, Andrew Young2, Nathan C Klapoetke1–3, Mike A Henninger1–3, Suhasa B Kodandaramaiah1–3,8, Masaaki Ogawa1–3, Shreshtha B Ramanlal9, Rachel C Bandler1, Brian D Allen1, Craig R Forest8, Brian Y Chow10, Xue Han9, Yingxi Lin2, Kay M Tye7, Botond Roska5, Jessica A Cardin4,11 & Edward S Boyden1–3
فقرة قد تكون خيط لتأثير الأدوية على البصر
Previous optogenetic hyperpolarizing proton pumps (Arch1, ArchT3, Mac1) and chloride pumps (eNpHR4, eNpHR3.0 (ref. 2)) have successfully inhibited volumes of approximately a cubic millimeter, but many neuroscience questions require the ability to suppress larger tissue volumes. A number of pharmacogenetic, chemical and genetic strategies have been used for this purpose5–7
المراجع
5. Ibañez-Tallon, I. et al. Tethering naturally occurring peptide toxins for cell-autonomous modulation of ion channels and receptors in vivo. Neuron 43, 305–311 (2004). 6. Armbruster, B.N., Li, X., Pausch, M.H., Herlitze, S. & Roth, B.L. Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand. Proc. Natl. Acad. Sci. USA 104, 5163–5168 (2007). 7. Kramer, R.H., Mourot, A. & Adesnik, H. Optogenetic pharmacology for control of native neuronal signaling proteins. Nat. Neurosci. 16, 816–823 (2013).
فقرة أخرى قد تكون خيط لتأثير الأدوية على البصر
A rebound burst of action potentials is common after illumination of cells expressing halorhodopsins18,27–30 or archaerhodopsins18,27,31, which respectively pump chloride inward and protons outward. A variety of possible mechanisms have been proposed, including hyperpolarization-activated Ih currents29,30,32 or changes in chloride reversal potential due to intracellular chloride accumulation33
http://home.uchicago.edu/~arij/journalclub/papers/2014_Chuong_et_al.pdf
